Description
Key facts
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:pH: 5.1 - 5.5Constituents: Citrate Buffer, 1.5% 2-butoxyethanol, 1% Hydrochloric acid
Product details:
Antigen Retrieval Buffer (100X Citrate Buffer) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. It is optimal for use with primary antibodies that require citrate buffer pretreatment.
This product contains detergent for emulsification of the paraffin.
1X Dilution:
The 100X stock solution should be diluted 100-fold with distilled water before use.
Protocol:
1. Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20 min.
2. Wash sections with hot tap water for 1 min.
3. Wash sections in buffer for 2 x 3 minutes.
4. Continue with IHC protocol as normal.
Other kits and reagents for IHC
Other antigen retrieval buffers include:
Tris-EDTA buffer pH 9.0 ab93684
EDTA buffer pH 8.0 ab93680
Tris buffer pH 10.0 ab93682
, or see the full list of
antigen retrieval buffers and enzymes
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient
