Biolegend, 100211, Alexa Fluor® 647 anti-mouse CD3 Antibody
25microg
Catalog Number: 100211
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | γδTCR-positive T-T hybridoma D1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-F, 3D IHC - Verified SB - Community verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2. |
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| Additional Product Notes | This product has been verified for IHC-F (Immunohistochemistry - frozen tissue sections) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page. |
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| Application References(PubMed link indicates BioLegend citation) | Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed Kang YA, et al. 2023. J Exp Med. 220:. PubMed Koop K, et al. 2023. Front Immunol. 14:1163198. PubMed Santini-González J, et al. 2022. Front Endocrinol (Lausanne). 13:989815. PubMed Hiraoka N, et al. 2011. Gastroenterology. 140:310. PubMed Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed Otano I, et al. 2021. Nat Commun. 12:7296. PubMed Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed Zeng Z, et al. 2022. Oncogene. :. PubMed Rassy D, et al. 2020. J Neuropathol Exp Neurol. 226:79. PubMed Xiong S 2013. J Immunol. 190:3267. PubMed Sluis T, et al. 2015. Clin Cancer Res. 21:781. PubMed Luck H, et al. 2019. Nat Commun. 10:3650. PubMed Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed Cecchinato V, et al. 2017. J Immunol. 198(1):184-195. PubMed Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed Ovadya Y, et al. 2018. Nat Commun. 9:5435. PubMed Ardehali R, et al. 2013. Proc Natl Acad Sci U S A. 110:3405. PubMed Agudo J et al. 2018. Immunity. 48(2):271-285 . PubMed Chen S, et al. 2015. Blood. 126: 103 - 112. PubMed Crinier A, et al. 2018. Immunity. 49:971. PubMed Bárcena C et al. 2019. EBioMedicine. 43:513-524 . PubMed Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed Chang H, et al. 2016. J Immunol. 197: 2473 - 2484. PubMed Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed |
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| RRID | AB_389323 (BioLegend Cat. No. 100209) |
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| Structure | Ig superfamily, CD3/TCR, 20 kD |
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| Distribution | Thymocytes (differentiation dependent), mature T cells, NK-T cells |
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| Function | Antigen recognition, TCR signal transduction, T cell activation |
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| Ligand/Receptor | Peptide antigen/MHC-complex |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263. |
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| Gene ID | 12502 |
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| UniProt | View information about CD3 on UniProt.org |
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| Clone | 17A2 |
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| Regulatory Status | RUO |
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| Other Names | T cell antigen receptor complex, T3 |
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| Isotype | Rat IgG2b, κ |
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