Biolegend, 100240, Alexa Fluor® 594 anti-mouse CD3 Antibody
100microg
Catalog Number: 100240
Send an RFQ.
Category
Loading…
| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | γδTCR-positive T-T hybridoma D1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-F - Quality tested FC, 3D IHC - Verified SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes | Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2. |
|---|
| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
|---|
| Application References(PubMed link indicates BioLegend citation) | Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
|---|
| Product Citations | Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed Mueller JPJ, et al. 2023. Front Immunol. 14:1034032. PubMed Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed Matsumoto R, et al. 2021. Front Pharmacol. 12:715752. PubMed Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed Liu M, et al. 2020. Nature. 587:115. PubMed Wong HS, et al. 2021. Cell. . PubMed Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed Prados A, et al. 2021. Nat Immunol. 22:510. PubMed Qi S et al. 2016. eLife. 5 pii: e14756. PubMed Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed Chung YM, et al. 2021. J Immunother Cancer. 9:. PubMed Palacio L, et al. 2019. Aging Cell. 18(4):e12971. PubMed Matryba P, et al. 2020. J Immunol. 1395:204. PubMed Wang X, et al. 2021. EMBO J. 40:e105926. PubMed Pantelidou C, et al. 2022. NPJ Breast Cancer. 8:102. PubMed Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed |
|---|
| RRID | AB_2563427 (BioLegend Cat. No. 100240) |
|---|
| Structure | Ig superfamily, CD3/TCR, 20 kD |
|---|
| Distribution | Thymocytes (differentiation dependent), mature T cells, NK-T cells |
|---|
| Function | Antigen recognition, TCR signal transduction, T cell activation |
|---|
| Ligand/Receptor | Peptide antigen/MHC-complex |
|---|
| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263. |
|---|
| Gene ID | 12502 |
|---|
| UniProt | View information about CD3 on UniProt.org |
|---|
| Clone | 17A2 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | T cell antigen receptor complex, T3 |
|---|
| Isotype | Rat IgG2b, κ |
|---|
