Biolegend, 101609, Alexa Fluor® 488 anti-mouse CD23 Antibody
100microg
Catalog Number: 101609
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser |
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| Blue Laser (488 nm) |
| Application Notes | The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, immunohistochemical staining of acetone-fixed frozen sections5, and spatial biology (IBEX)7,8. |
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| Application References(PubMed link indicates BioLegend citation) | Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP) Rao M, et al. 1987. J. Immunol. 138:1845. (Block) Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block) Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block) Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC) Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Weingartner E, et al. 2017. J Immunol Methods. 1:441. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed |
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| RRID | AB_493362 (BioLegend Cat. No. 101609) |
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| Structure | C-lectin family, type II transmembrane glycoprotein, 45 kD |
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| Distribution | B cells, follicular dendritic cells |
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| Function | Regulates B cell activation |
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| Ligand/Receptor | IgE, CD19/CD21/CD81 |
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| Cell Type | B cells, Dendritic cells |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Fc Receptors |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Delespesse G, et al. 1992. Immunol. Rev. 125:77. 3. Flores-Romo L, et al. 1993. Science 261:1038. |
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| Gene ID | 14128 |
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| UniProt | View information about CD23 on UniProt.org |
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| Clone | B3B4 |
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| Regulatory Status | RUO |
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| Other Names | FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6 |
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| Isotype | Rat IgG2a, κ |
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