Biolegend, 396436, Brilliant Violet 650™ anti-human/mouse Granzyme B Recombinant Antibody
100tests
Catalog Number: 396436
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Recombinant |
| Host Species | Rat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
Answer:Brilliant Violet 650™ Anti-Human/Mouse Granzyme B Recombinant Antibody, 396436.
Answer:Human/mouse Granzyme B; BV650; Intracellular flow.
Answer:Brilliant Violet 650™ anti-human/mouse Granzyme B is a fluorescent recombinant antibody used in flow-cytometric analysis of cytotoxic lymphocytes.
Brilliant Violet 650™ anti-human/mouse Granzyme B is a fluorescent recombinant antibody used in flow-cytometric analysis of cytotoxic lymphocytes. Granzyme B is an intracellular serine protease associated with activated cytotoxic T cells and natural killer cells. The BV650 label is designed for violet-laser excitation and helps incorporate this intracellular effector marker into complex immunophenotyping panels. Samples generally require appropriate fixation and permeabilization before intracellular detection. The antibody is useful in studies of cytotoxic immune responses, activation, and functional cell-state changes in human and mouse samples. Panel compensation, titration, and compatible fixation chemistry should be established for the full assay.
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 10^6 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). |
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| RRID | AB_3662293 (BioLegend Cat. No. 396435) AB_3662293 (BioLegend Cat. No. 396436) |
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| Structure | 32 kD serine protease |
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| Distribution | Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells |
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| Function | Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46. |
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| Interaction | Caspase-3 |
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| Ligand/Receptor | Mannose-6-phosphate receptor |
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| Cell Type | Neutrophils, T cells |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroscience |
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| Molecular Family | Enzymes and Regulators, Proteases |
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| Antigen References | Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. Wagner C, et al. 2008. Mol. Immunol. 45:1761. |
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| Gene ID | 300214939 |
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| UniProt | View information about Granzyme B on UniProt.org |
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| Clone | QA18A28 |
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| Regulatory Status | RUO |
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| Other Names | Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase |
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| Isotype | Rat IgG1, κ |
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