Biolegend, 372710, Brilliant Violet 421™ anti-human TIGIT (VSTM3) Antibody
100tests
Catalog Number: 372710
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant Human TIGIT. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
Answer:Brilliant Violet 421™ Anti-Human TIGIT Antibody, 372710.
Answer:Human TIGIT; BV421; Flow cytometry.
Answer:Brilliant Violet 421™ anti-human TIGIT is a fluorescent antibody for flow-cytometric detection of TIGIT, an inhibitory immune receptor expressed on selected T cells and natural killer cells.
Brilliant Violet 421™ anti-human TIGIT is a fluorescent antibody for flow-cytometric detection of TIGIT, an inhibitory immune receptor expressed on selected T cells and natural killer cells. TIGIT is studied in immune regulation, exhaustion-associated phenotypes, and the balance of activating and inhibitory pathways. The BV421 label is excited by a violet laser and supports multicolor surface-staining panels that evaluate TIGIT alongside lineage and differentiation markers. This research antibody can help characterize immune-cell populations in oncology, infection, and immunology studies. Data interpretation should account for marker combinations rather than treating TIGIT positivity as a standalone functional diagnosis. Confirm the full SKU's clone and package size during procurement.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).Additional reported applications (for the relevant formats) include: Blocking1. |
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| Application References(PubMed link indicates BioLegend citation) | Stamm H, et al. 2018. Oncogene. Pubmed |
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| Product Citations | Beck C, et al. 2023. Biomedicines. 11:. PubMed Lorvik KB, et al. 2021. Front Immunol. 12:744155. PubMed Sun W, et al. 2023. Nature. 614:309. PubMed Xu L, et al. 2021. Front Oncol. 11:686156. PubMed Kuo HH, et al. 2018. Immunity. 48:1183. PubMed Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed Shi X, et al. 2022. NPJ Precis Oncol. 6:58. PubMed |
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| RRID | AB_2632924 (BioLegend Cat. No. 372709) AB_2632925 (BioLegend Cat. No. 372710) |
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| Structure | 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif. |
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| Distribution | Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells. |
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| Function | Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy. |
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| Ligand/Receptor | CD155 (PVR), CD112 (PVRL2, NECTIN-2). |
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| Cell Type | NK cells, T cells, Tfh, Tregs |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction |
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| Molecular Family | Adhesion Molecules, Immune Checkpoint Receptors |
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| Antigen References | 1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858. 2. Yu X, et al. 2009. Nat. Immunol. 10:48. 3. Johnston R, et al. 2014. Cancer Cell. 26:923. |
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| Gene ID | 201633 |
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| UniProt | View information about TIGIT on UniProt.org |
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| Clone | A15153G |
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| Regulatory Status | RUO |
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| Other Names | T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM |
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| Isotype | Mouse IgG2a, κ |
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