Biolegend, 331926, Brilliant Violet 605™ anti-human CD335 (NKp46) Antibody
100tests
Catalog Number: 331926
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | NKp46-Fc fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
Answer:Brilliant Violet 605™ Anti-Human CD335 (NKp46) Antibody, 331926.
Answer:Human NKp46; BV605; Flow cytometry.
Answer:Brilliant Violet 605™ anti-human CD335 recognizes NKp46, an activating receptor associated with human natural killer cells and selected innate lymphoid cell populations.
Brilliant Violet 605™ anti-human CD335 recognizes NKp46, an activating receptor associated with human natural killer cells and selected innate lymphoid cell populations. The BV605 fluorophore is excited by a violet laser and supports inclusion of NKp46 in multicolor flow-cytometry panels. Measuring CD335 together with other lineage markers can help distinguish NK-cell subsets and examine changes in their phenotype across experimental conditions. The reagent is intended for research immunophenotyping rather than direct clinical diagnosis. Staining and compensation must be optimized for the specific panel, fluorophore combinations, and specimen preparation. Package size and antibody clone are not inferred from the conjugate family.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | Clone 9E2 has been shown to block NK activation through NKp46.6 |
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| Application References(PubMed link indicates BioLegend citation) | Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. Kalberer CP, et al. 2003. Blood 102:127. Chen Y, et al. 2007. J. Immunol. 179:2766. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Achdout H. et al. 2010. J. Virol. 84:3993. |
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| Product Citations | Mayassi T, et al. 2019. Cell. 176:967. PubMed Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed |
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| RRID | AB_2562310 (BioLegend Cat. No. 331925) AB_2563855 (BioLegend Cat. No. 331926) |
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| Structure | Type I membrane glycoprotein (46 kD) |
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| Distribution | Expressed on resting and activated NK cells |
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| Cell Type | NK cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147. 2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. 3. Sivori S. 1999. Eur. J. Immunol. 29:1656. |
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| Gene ID | 9437 |
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| UniProt | View information about CD335 on UniProt.org |
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| Clone | 9E2 |
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| Regulatory Status | RUO |
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| Other Names | NKp46, NCR1 |
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| Isotype | Mouse IgG1, κ |
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