Biolegend, 103123, Alexa Fluor® 647 anti-mouse CD45 Antibody
25microg
Catalog Number: 103123
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse thymus or spleen |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICC, 3D IHC - Verified SB - Community verified IHC, SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for other applications. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45. Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164). |
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| Additional Product Notes | For use in spatial biology, this antibody has been demonstrated for use in immunohistochemistry using IBEX (Reported in the literature, not verified in house) and the NanoString GeoMx® Digital Spatial Profiler. IBEX: Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). NanoString GeoMx®: This product has been verified for IHC-F (Immunohistochemistry - frozen tissue sections) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page. |
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| Application References(PubMed link indicates BioLegend citation) | Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC) Seaman WE. 1983. J. Immunol. 130:1713. (CMCD) Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC) Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed Liu F, et al. 2012. Blood. 119:3295. PubMed Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Osterloh A, et al. 2016. Infect Immun . . PubMed Moderzynski K, et al. 2016. PLoS Negl Trop Dis. . PubMed Chen W, et al. 2023. JCI Insight. 8:. PubMed Iguchi A, et al. 2023. iScience. 26:106375. PubMed Yan Y, et al. 2021. Immunity. 54:499. PubMed Yamamoto A, et al. 2022. Arthritis Rheumatol. 74:860. PubMed Frederico B, et al. 2022. Dev Cell. 57:1957. PubMed Cho J, et al. 2016. Nat Commun. 7:13373. PubMed Donato C, et al. 2020. Cell Reports. 32(10):108105. PubMed Boal-Carvalho I, et al. 2020. EMBO Rep. 21:e50421. PubMed Hu X, et al. 2016. Nat Commun. 7:13095. PubMed Moore SM, et al. 2019. Transl Psychiatry. 9:24. PubMed Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed Hu K, et al. 2015. PLoS One. 10: 0137123. PubMed Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed Sutton NR, et al. 2019. Arterioscler Thromb Vasc Biol. 40:61. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Fang EF, et al. 2019. Nat Neurosci. 22:401. PubMed Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed Piedrafita G, et al. 2020. Nat Commun. 11:1429. PubMed Uceda-Castro R, et al. 2022. Cell Rep Med. 3:100821. PubMed Donato C, et al. 2021. STAR Protocols. 2(2):100480. PubMed Pineda CM, et al. 2019. J Cell Biol. 218:3212. PubMed Kang MH, et al. 2020. Nat Commun. 3.186805556. PubMed Kim S, et al. 2022. Redox Biol. 54:102347. PubMed Levesque S, et al. 2019. Oncoimmunology. 8:e1657375. PubMed Kuhn JA, et al. 2021. Elife. 10:. PubMed Simões FC, et al. 2020. Nat Commun. 0.875. PubMed Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed Huang L, et al. 2018. Exp Neurol. 300:41. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Wang S, et al. 2021. Commun Biol. 22:. PubMed Shibuya M, et al. 2021. iScience. 24:103131. PubMed de Winde CM, et al. 2021. J Cell Sci. 134: . PubMed Ovadya Y, et al. 2018. Nat Commun. 9:5435. PubMed Yomtoubian S, et al. 2020. Cell Reports. 30(3):755-770.e6.. PubMed Lu YJ, et al. 2021. Cell Rep. 36:109696. PubMed Thacker VV, et al. 2020. Elife. 9:00. PubMed Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Bakalar MH et al. 2018. Cell. 174(1):131-142 . PubMed Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed Xiong J et al. 2018. Molecular cell. 69(4):689-698 . PubMed Williams IM, et al. 2018. J Clin Invest. 128:699. PubMed Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed Oni TE, et al. 2020. J Exp Med. :217. PubMed Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed |
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| RRID | AB_493534 (BioLegend Cat. No. 103123) AB_493533 (BioLegend Cat. No. 103124) |
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| Structure | Protein tyrosine phosphatase (PTP) family, 180-240 kD |
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| Distribution | All hematopoietic cells except mature erythrocytes and platelets |
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| Function | Phosphatase, T and B cell activation |
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| Ligand/Receptor | Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1 |
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| Cell Type | B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs |
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| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851. |
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| Gene ID | 19264 |
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| UniProt | View information about CD45 on UniProt.org |
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| Clone | 30-F11 |
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| Regulatory Status | RUO |
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| Other Names | T200, Ly-5, LCA |
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| Isotype | Rat IgG2b, κ |
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