Biolegend, 103809, Alexa Fluor® 488 anti-mouse CD49e Antibody
25microg
Catalog Number: 103809
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | (C57BL/6 x A/J)F1 mouse mast cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of acetone-fixed frozen sections, blocking of cell-cell adhesion1-5, inhibition of TNF-ß1 costimulated T cell proliferation3, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103817 and 103818). |
| Application References(PubMed link indicates BioLegend citation) | Kinashi T, et al. 1994. Blood Cells 20:25. (Block) Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block) Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim)) Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block) Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block) Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC) Cuklerman E, et al. 2001. Science 294:1708. (IHC) Jia W, et al. 2005. Blood 106:3854. (FC) |
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| Product Citations | Yan Q, et al. 2017. PLoS One. 12(6):e0179033. PubMed Duru N, et al. 2016. Sci Rep. 6:39572. PubMed Le AH, et al. 2021. Journal of Cell Biology. 220(9):. PubMed |
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| RRID | AB_528839 (BioLegend Cat. No. 103810) |
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| Structure | Integrin family, associates with integrin β1, 135 kD |
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| Distribution | Thymocytes, activated T cells, mast cells |
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| Function | Adhesion, costimulation |
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| Ligand/Receptor | Fibronectin |
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| Cell Type | Mast cells, Mesenchymal Stem Cells, T cells, Thymocytes, Tregs |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kinashi T, et al. 1994 Blood Cells 20:25. 3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365. |
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| Gene ID | 16402 |
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| UniProt | View information about CD49e on UniProt.org |
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| Clone | 5H10-27(MFR5) |
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| Regulatory Status | RUO |
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| Other Names | α5 integrin, VLA-5 α chain, Integrin α5, Fnra, Itga5 |
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| Isotype | Rat IgG2a, κ |
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