Biolegend, 104311, Alexa Fluor® 488 anti-mouse/rat CD61 Antibody
25microg
Catalog Number: 104311
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| Verified Reactivity | Mouse, Rat |
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| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | Vitronectin receptor protein from the mouse T-cell hybridoma 2B4. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser |
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| Blue Laser (488 nm) |
| Application Notes | The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104325 and 104326). |
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| Application References(PubMed link indicates BioLegend citation) | Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block) Piali L, et al. 1995. J. Cell Biol. 130:451. (Block) Ashkar S, et al. 2000. Science 287:860. (Block) Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block) Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ) Carlson TR, et al. 2008.135:2193. PubMed Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed |
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| Product Citations | Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed Jeanes A, et al. 2012. J Biol Chem. 287:24103. PubMed Oliver C, et al. 2012. Genes Dev. 26:1086. PubMed Del Barco Barrantes I, et al. 2018. Stem Cell Reports. 10:257. PubMed Gross KM, et al. 2019. Cell Rep. 28:394. PubMed |
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| RRID | AB_2128907 (BioLegend Cat. No. 104311) |
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| Structure | Integrin family, 110 kD |
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| Distribution | Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells |
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| Function | Attachment to matrix |
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| Ligand/Receptor | Fibrinogen, von Willebrand factor, fibronectin |
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| Cell Type | B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Phillips DR, et al. 1991. Cell. 65:359. 3. Felding-Habermann B, et al. 1993. Curr Opinion Cell Biol. 5:864. |
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| Gene ID | 1641629302 |
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| UniProt | View information about CD61 on UniProt.org |
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| Clone | 2C9.G2 (HMβ3-1) |
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| Regulatory Status | RUO |
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| Other Names | β3 integrin, Integrin β3 chain, gpIIIa |
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| Isotype | Armenian Hamster IgG |
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