Biolegend, 104312, Alexa Fluor® 488 anti-mouse/rat CD61 Antibody
100microg
Catalog Number: 104312
Send an RFQ.
Category
Loading…
| Verified Reactivity | Mouse, Rat |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | Vitronectin receptor protein from the mouse T-cell hybridoma 2B4. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser |
|---|
| Blue Laser (488 nm) |
| Application Notes | The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104325 and 104326). |
|---|
| Application References(PubMed link indicates BioLegend citation) | Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block) Piali L, et al. 1995. J. Cell Biol. 130:451. (Block) Ashkar S, et al. 2000. Science 287:860. (Block) Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block) Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ) Carlson TR, et al. 2008.135:2193. PubMed Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed |
|---|
| Product Citations | Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed Jeanes A, et al. 2012. J Biol Chem. 287:24103. PubMed Oliver C, et al. 2012. Genes Dev. 26:1086. PubMed Del Barco Barrantes I, et al. 2018. Stem Cell Reports. 10:257. PubMed Gross KM, et al. 2019. Cell Rep. 28:394. PubMed |
|---|
| RRID | AB_2128907 (BioLegend Cat. No. 104311) |
|---|
| Structure | Integrin family, 110 kD |
|---|
| Distribution | Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells |
|---|
| Function | Attachment to matrix |
|---|
| Ligand/Receptor | Fibrinogen, von Willebrand factor, fibronectin |
|---|
| Cell Type | B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells |
|---|
| Biology Area | Cell Adhesion, Cell Biology, Immunology |
|---|
| Molecular Family | Adhesion Molecules, CD Molecules |
|---|
| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Phillips DR, et al. 1991. Cell. 65:359. 3. Felding-Habermann B, et al. 1993. Curr Opinion Cell Biol. 5:864. |
|---|
| Gene ID | 1641629302 |
|---|
| UniProt | View information about CD61 on UniProt.org |
|---|
| Clone | 2C9.G2 (HMβ3-1) |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | β3 integrin, Integrin β3 chain, gpIIIa |
|---|
| Isotype | Armenian Hamster IgG |
|---|
