Biolegend, 105315, Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2) Antibody
25microg
Catalog Number: 105315
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse thymus or spleen |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352). |
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| Application References(PubMed link indicates BioLegend citation) | Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete) Seaman WE. 1983. J. Immunol. 130:1713. (Deplete) Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim) Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC) Hardy B, et al. 2005. Int. Immunol. 17:615. Drobyski W, et al. 1996. Blood 87:5355. (Deplete) Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed Sungur CM, et al. 2013. PNAS. 110:7401. PubMed |
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| Product Citations | Zheng P, et al. 2023. Stroke Vasc Neurol. . PubMed Yeh CH, et al. 2022. Immunity. 55:272. PubMed Todd E, et al. 2011. J Immunol. 187:3015. PubMed Wu N, et al. 2020. Cell Rep. 30:1129. PubMed Amano C, et al. 2015. PLoS One. 10: 0137207. PubMed Kretschmer S, et al. 2013. PLoS One. 8:e55201. PubMed Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed Armaka M, et al. 2022. Genome Med. 14:78. PubMed García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed Schliehe C, et al. 2012. J Virol. 139:3442. PubMed Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed Armaka M, et al. 2018. Nat Commun. 9:618. PubMed Mohammed RN, et al. 2019. Sci Rep. 4.185416667. PubMed Prasai A, et al. 2017. Stem Cell Rev.. 10.1007/s12015-017-9721-9. PubMed |
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| RRID | AB_492887 (BioLegend Cat. No. 105315) AB_492886 (BioLegend Cat. No. 105316) |
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| Structure | Ig superfamily, 25-35 kD |
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| Distribution | Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice |
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| Function | Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells |
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| Ligand/Receptor | CD45 |
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| Cell Type | Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Craig W, et al. 1993. J. Exp. Med. 177:1331. 3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1. 4. Mayani H, et al. 1994. Blood 83:2410. |
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| Gene ID | 21838 |
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| UniProt | View information about CD90.2 on UniProt.org |
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| Clone | 30-H12 |
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| Regulatory Status | RUO |
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| Other Names | Thy-1.2 |
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| Isotype | Rat IgG2b, κ |
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