Biolegend, 105609, Alexa Fluor® 488 anti-mouse CD102 Antibody
25microg
Catalog Number: 105609
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | COS cells transfected with mouse ICAM-2 cDNA |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105608). |
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| Application References(PubMed link indicates BioLegend citation) | Xu H, et al. 1996. J. Immunol. 156:4909. Gerwin N, et al. 1999. Immunity 10:9. |
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| Product Citations | Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Halai K, et al. 2014. J Cell Sci. 127:620. PubMed Watari K, et al. 2020. Commun Biol. 3:107. PubMed Sung SJ, et al. 2020. Bio Protoc. 10:e3663. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Reimer D, et al. 2020. Cell Reports. 32(6):108030. PubMed |
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| RRID | AB_2264501 (BioLegend Cat. No. 105609) |
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| Structure | Ig superfamily, 55 kD |
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| Distribution | Vascular endothelial cells, some resting lymphocytes, monocytes, platelets |
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| Function | Lymphocyte recirculation, costimulation |
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| Ligand/Receptor | LFA-1, Mac-1 |
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| Cell Type | Endothelial cells, Lymphocytes, Monocytes, Platelets |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017. 3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129. |
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| Gene ID | 15896 |
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| UniProt | View information about CD102 on UniProt.org |
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| Clone | 3C4 (MIC2/4) |
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| Regulatory Status | RUO |
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| Other Names | ICAM-2 |
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| Isotype | Rat IgG2a, κ |
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