Biolegend, 105709, Alexa Fluor® 488 anti-mouse CD106 Antibody
25microg
Catalog Number: 105709
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse preadipose cell line PA6 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality testes IHC-F - Verified SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, immunoprecipitation1 , and spacial biology (IBEX)11,12. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 & 105728). |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC) Kinashi T, et al. 1994. Blood Cells 20:25. (Block) Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC) Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC) Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed Monnier J, et al. 2012. J. Immunol. 189:956. PubMed Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Ulyanova T, Phelps S, Papayannopoulou T 2016. Blood. 128: 1756 - 1765. PubMed Verhoeven J, et al. 2022. Methods Mol Biol. 2572:45. PubMed Alexandre YO, et al. 2022. STAR Protoc. 3:101923. PubMed Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed |
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| RRID | AB_493427 (BioLegend Cat. No. 105710) |
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| Structure | Ig superfamily, 47 kD |
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| Distribution | Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells |
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| Function | Adhesion |
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| Ligand/Receptor | VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin) |
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| Cell Type | Dendritic cells, Endothelial cells, Mesenchymal Stem Cells |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. 3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767. 4. Koni PA, et al. 2001. J. Exp. Med. 193:741. |
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| Gene ID | 22329 |
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| UniProt | View information about CD106 on UniProt.org |
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| Clone | 429 (MVCAM.A) |
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| Regulatory Status | RUO |
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| Other Names | VCAM-1, INCAM-110 |
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| Isotype | Rat IgG2a, κ |
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