Biolegend, 105815, Alexa Fluor® 488 anti-mouse CD117 (c-Kit) Antibody
25microg
Catalog Number: 105815
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse bone marrow mast cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality testedIHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity. |
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| Application References(PubMed link indicates BioLegend citation) | Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC) Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC) Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC) Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Obata Y, et al. 2014. Nat Commun. 5:5715. PubMed Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed Xiong H, et al. 2021. Theranostics. 11:1594. PubMed Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed |
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| RRID | AB_493473 (BioLegend Cat. No. 105815) AB_493472 (BioLegend Cat. No. 105816) |
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| Structure | Ig superfamily, 145 kD |
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| Distribution | Hematopoietic stem cells, AML, mast cells |
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| Function | Growth factor receptor, tyrosine kinase |
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| Ligand/Receptor | Stem Cell Factor (SCF) |
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| Cell Type | Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells |
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| Biology Area | Immunology, Stem Cells |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5. |
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| Gene ID | 16590 |
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| UniProt | View information about CD117 on UniProt.org |
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| Clone | 2B8 |
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| Regulatory Status | RUO |
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| Other Names | c-KIT, Stem Cell Factor Receptor (SCFR) |
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| Isotype | Rat IgG2b, κ |
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