Biolegend, 105828, Brilliant Violet 421™ anti-mouse CD117 (c-Kit) Antibody
500microl
Catalog Number: 105828
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse bone marrow mast cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested SB - Reported in the literature, not verified in house |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC) Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC) Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC) Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Tan J, et al. 2021. iScience. 24(8):102835. PubMed Kaltenbach L, et al. 2023. Nat Immunol. 24:915. PubMed Feng X, et al. 2023. Nat Commun. 14:3208. PubMed Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed Issa N, et al. 2023. Leukemia. :. PubMed Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Liu L, et al. 2022. Cancers (Basel). 14:. PubMed Thiele K, et al. 2015. Am J Pathol. Available online 5 August 2015. PubMed Wu W, et al. 2014. Proc Natl Acad Sci U S A. 111:4221. PubMed Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed Tyrkalska SD, et al. 2019. Immunity. 51:50. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed Wei Q, et al. 2020. Dev Cell. 53:503. PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed Celik H, et al. 2018. Cancer Cell. 34:741. PubMed Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Gao X, et al. 2021. Nature. 589:591. PubMed Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed Iwanami N, et al. 2020. iScience. 23:101260. PubMed Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed Boulch M, et al. 2021. Sci Immunol. 6:. PubMed Gross KM, et al. 2019. Cell Rep. 28:394. PubMed Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed Strattan E, et al. 2020. Frontiers in Immunology. 2.131944444. PubMed Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed Abdel Malik R, et al. 2017. Circ Res. 120:99. PubMed |
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| RRID | AB_10898120 (BioLegend Cat. No. 105827) AB_11204256 (BioLegend Cat. No. 105828) |
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| Structure | Ig superfamily, 145 kD |
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| Distribution | Hematopoietic stem cells, AML, mast cells |
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| Function | Growth factor receptor, tyrosine kinase |
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| Ligand/Receptor | Stem Cell Factor (SCF) |
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| Cell Type | Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells |
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| Biology Area | Immunology, Stem Cells |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5. |
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| Gene ID | 16590 |
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| UniProt | View information about CD117 on UniProt.org |
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| Clone | 2B8 |
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| Regulatory Status | RUO |
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| Other Names | c-KIT, Stem Cell Factor Receptor (SCFR) |
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| Isotype | Rat IgG2b, κ |
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