Biolegend, 106003, Biotin anti-mouse CD123 Antibody
50microg
Catalog Number: 106003
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | CTLL cells transfected with the mouse IL-3Rα chain |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106004) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. |
| Application References(PubMed link indicates BioLegend citation) | Ichihara, et al. 1995. EMBO J. 14:939. (FC) Mueller DL, et al. 1994. J. Immunol. 153:3014. (FC) Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) |
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| Product Citations | Knox T, et al. 2019. Sci Rep. 9:6136. PubMed |
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| RRID | AB_2124404 (BioLegend Cat. No. 106003) |
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| Structure | Ig superfamily, associates with CDw131 (common β chain) or IL-3 receptor β chain, 70 kD |
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| Distribution | Hematopoietic progenitors, basophils, mast cells, megakaryocytes |
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| Function | Proliferation, differentiation of hematopoietic cells |
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| Ligand/Receptor | IL-3 |
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| Cell Type | Basophils, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Megakaryocytes |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Miyajima A, et al. 1993. Blood 82:1960. 3. Itoh N, et al. 1990. Science 247:324. |
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| Gene ID | 16188 |
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| UniProt | View information about CD123 on UniProt.org |
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| Clone | 5B11 |
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| Regulatory Status | RUO |
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| Other Names | IL-3 Receptor α chain, IL-3Rα |
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| Isotype | Rat IgG2a, κ |
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