Biolegend, 106304, Biotin anti-mouse CD152 Antibody
500microg
Catalog Number: 106304
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | Mouse CTLA-4-mouse IgG2a fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation, in vitro and in vivo blocking1-4 of ligand binding, and as ELISA capture antibody5. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106327). |
| Application References(PubMed link indicates BioLegend citation) | Walunas TL, et al. 1994. Immunity 1:405. (Block, IP) Cilio CM, et al. 1998. J. Exp. Med. 188:1239. (Block) Issazadeh S, et al. 1999. J. Immunol. 162:761. (Block) McCoy K, et al. 1997. J. Exp. Med. 186:183. (Block) Hsu HC, et al. 2007. J. Immunol. 178:5357. (ELISA Capture) Sugita S, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:5783. PubMed |
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| Product Citations | Zenke S, et al. 2020. Immunity. 52(2):313-327. PubMed Nakazawa Y, et al. 2021. Elife. 10:. PubMed |
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| RRID | AB_313252 (BioLegend Cat. No. 106303) AB_313253 (BioLegend Cat. No. 106304) |
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| Structure | Ig superfamily, 33 kD |
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| Distribution | Activated T cells and B cells |
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| Function | Negative regulator of T cell activation |
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| Ligand/Receptor | CD80 (B7-1), CD86 (B7-2) |
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| Cell Type | B cells, T cells, Tregs |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Immune Checkpoint Receptors |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Allison JP, et al. 1995. Science 270:932. 3. Waterhouse P, et al. 1995. Science 270:985. 4. Linsley PS, et al. 1991. J. Exp. Med. 174:561. |
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| Gene ID | 12477 |
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| UniProt | View information about CD152 on UniProt.org |
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| Clone | UC10-4B9 |
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| Regulatory Status | RUO |
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| Other Names | Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56 |
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| Isotype | Armenian Hamster IgG |
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