Biolegend, 108112, APC anti-mouse Ly-6A/E (Sca-1) Antibody
100microg
Catalog Number: 108112
Loading…
| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | IL-2-dependent mouse T-cell line (CTL-L) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. |
| Concentration | 0.2 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Excitation Laser | Red Laser (633 nm) |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes |
|---|
| The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9. The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 |
| Application References(PubMed link indicates BioLegend citation) | Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP) Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB) Codias EK, et al. 1990. J. Immunol. 144:2197. Malek TR, et al. 1986. J. Exp. Med. 164:709. Codias EK, et al. 1990. J. Immunol. 145:1407. Ivanov V, et al. 1994. J. Immunol. 153:2394. Karlhofer FM, et al. 1991. J. Immunol. 146:3662. Fleming T, et al. 1994. J. Immunol. 153:1955. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC) Umland O, et al. 2007. J. Immunol. 178:4147. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed Pronk CJ, et al. 2011. J. Exp Med. PubMed English A, et al. 2000. J. Immunol. 165:3763. (IHC) Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed |
|---|
| Product Citations | Feng X, et al. 2023. Nat Commun. 14:3208. PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Leinroth AP, et al. 2022. Cell Rep. 39:110785. PubMed Moiseeva V, et al. 2023. Nature. 613:169. PubMed Tang Y, et al. 2022. Dev Cell. 57:480. PubMed Li DD, et al. 2022. Cell Host Microbe. 30:530. PubMed Mikami Y, et al. 2021. Immunity. 54(3):514-525.e6. PubMed Umland O, et al. 2007. J Immunol . 178:4147. PubMed Ju W, et al. 2020. J Biomed Sci. 27:91. PubMed Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed Shi Y, et al. 2020. Med Sci Monit. 26:e923328. PubMed Bo Yu et al. 2018. Cell stem cell. 23(2):193-209 . PubMed Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed Larouche JA, et al. 2021. eLife. 0.416666666666667. PubMed Nguyen N, et al. 2022. iScience. 25:103679. PubMed Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed Liu X, et al. 2021. eLife. 0.416666666666667. PubMed Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Yin N et al. 2019. Cancer Cell. 36(2):156-167 . PubMed Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed Endo Y, et al. 2020. FASEB J. 34:16086. PubMed Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed De S, et al. 2018. Development. 145. PubMed Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed Shcherbina A, et al. 2020. Cell Rep. 32:107964. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Jungwirth N et al. 2018. Journal of molecular neuroscience : MN. 65(1):60-73 . PubMed Hou M, et al. 2020. Stem Cell Res Ther. 11:55. PubMed Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed Ferreirinha P, et al. 2022. Development. 149: . PubMed Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed Ikram M, et al. 2018. Oncotarget. 9:2058. PubMed Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed Mizbani A, et al. 2016. Development. 143: 4137 - 4148. PubMed Ding P, et al. 2022. Bone Res. 10:42. PubMed Gentek R, et al. 2018. Immunity. 48:1160. PubMed Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed Luo H, et al. 2020. Cancer Cell. 36(6):645-659.e8.. PubMed Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed Bastianutto C, et al. 2007. Cancer Res. 67:10112. PubMed Glotzbach J, et al. 2011. PLoS One. 6:e21211. PubMed Luo H, et al. 2022. Mol Cell. 82:833. PubMed Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed Wong J, et al. 2016. Sci Signal. 9: ra103. PubMed Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed Zhang Y, et al. 2008. Mol Cell Biol. 28:1688. PubMed Thomson CA, et al. 2018. J Immunol. 201:215. PubMed Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed Vogiatzi A, et al. 2021. Mol Cell Biol. 41:e0014921. PubMed |
|---|
| RRID | AB_313348 (BioLegend Cat. No. 108111) AB_313349 (BioLegend Cat. No. 108112) |
|---|
| Structure | Ly-6 multigene family, 18 kD |
|---|
| Distribution | Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells |
|---|
| Function | Regulates B and T cell responses |
|---|
| Cell Type | B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells |
|---|
| Biology Area | Immunology, Stem Cells |
|---|
| Antigen References | 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709. |
|---|
| Gene ID | 110454 |
|---|
| UniProt | View information about Ly-6A/E on UniProt.org |
|---|
| Clone | D7 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Sca-1 |
|---|
| Isotype | Rat IgG2a, κ |
|---|
