Biolegend, 108115, Alexa Fluor® 488 anti-mouse Ly-6A/E (Sca-1) Antibody
25microg
Catalog Number: 108115
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | IL-2-dependent mouse T-cell line (CTL-L) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9. The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 |
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| Application References(PubMed link indicates BioLegend citation) | Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP) Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB) Codias EK, et al. 1990. J. Immunol. 144:2197. Malek TR, et al. 1986. J. Exp. Med. 164:709. Codias EK, et al. 1990. J. Immunol. 145:1407. Ivanov V, et al. 1994. J. Immunol. 153:2394. Karlhofer FM, et al. 1991. J. Immunol. 146:3662. Fleming T, et al. 1994. J. Immunol. 153:1955. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC) Umland O, et al. 2007. J. Immunol. 178:4147. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed Pronk CJ, et al. 2011. J. Exp Med. PubMed English A, et al. 2000. J. Immunol. 165:3763. (IHC) Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed |
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| Product Citations | Kang YA, et al. 2023. J Exp Med. 220:. PubMed Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed Shen YC, et al. 2021. Nat Commun. 12:3876. PubMed Zhuo Y, et al. 2018. Oncol Rep. 40:2147. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Parmar N, et al. 2021. PLoS Pathog. 17:e1009476. PubMed Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed |
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| RRID | AB_493270 (BioLegend Cat. No. 108115) AB_493269 (BioLegend Cat. No. 108116) |
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| Structure | Ly-6 multigene family, 18 kD |
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| Distribution | Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells |
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| Function | Regulates B and T cell responses |
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| Cell Type | B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells |
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| Biology Area | Immunology, Stem Cells |
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| Antigen References | 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709. |
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| Gene ID | 110454 |
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| UniProt | View information about Ly-6A/E on UniProt.org |
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| Clone | D7 |
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| Regulatory Status | RUO |
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| Other Names | Sca-1 |
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| Isotype | Rat IgG2a, κ |
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