Biolegend, 108125, APC/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody
25microg
Catalog Number: 108125
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | IL-2-dependent mouse T-cell line (CTL-L) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions. |
| Concentration | 0.2 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9. The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 |
| Application References(PubMed link indicates BioLegend citation) | Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP) Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB) Codias EK, et al. 1990. J. Immunol. 144:2197. Malek TR, et al. 1986. J. Exp. Med. 164:709. Codias EK, et al. 1990. J. Immunol. 145:1407. Ivanov V, et al. 1994. J. Immunol. 153:2394. Karlhofer FM, et al. 1991. J. Immunol. 146:3662. Fleming T, et al. 1994. J. Immunol. 153:1955. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC) Umland O, et al. 2007. J. Immunol. 178:4147. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed Pronk CJ, et al. 2011. J. Exp Med. PubMed English A, et al. 2000. J. Immunol. 165:3763. (IHC) Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed |
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| Product Citations | Barros-Silva JD, et al. 2018. Cell Rep. 25:3504. PubMed Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed Kurbangaleeva S, et al. 2023. Curr Issues Mol Biol. 45:2431. PubMed Gautheron F, et al. 2023. Cell Death Discov. 9:117. PubMed Lütge M, et al. 2023. Nat Immunol. . PubMed Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed Xin DQ, et al. 2022. Neural Regen Res. 17:2238. PubMed Corna G, et al. 2016. J Immunol. 197: 1914 - 1925. PubMed Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed Kobayashi T, et al. 2019. Cell. 176:982. PubMed Chen X, et al. 2021. Theranostics. 11:4655. PubMed Fu X, et al. 2015. J Biol Chem. 290: 26445 - 26456. PubMed Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed Valds-Mora F, et al. 2021. Cell Reports. 35(2):108945. PubMed Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed Grandl G, et al. 2016. Mol Metab. 5:937-947. PubMed JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed McAlpine CS, et al. 2021. Nature. 595:701. PubMed Gomzikova MO, et al. 2020. Sci Rep. 10:10740. PubMed Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Helbling PM, et al. 2019. Cell Rep. 29:3313. PubMed Hermetet F, et al. 2019. Nat Commun. 10:523. PubMed Ye H, et al. 2021. Cancer Discov. 0.805555556. PubMed Agarwal P, et al. 2019. Cell Stem Cell. 24:769. PubMed Koyama T, et al. 2022. Curr Issues Mol Biol. 44:3146. PubMed Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed Ozaki M, et al. 2021. J Stem Cells Regen Med. 16:50. PubMed Jie Z, et al. 2014. J Immunol. 192:3289. PubMed Rai S, et al. 2022. Nat Commun. 13:5346. PubMed Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed Hu B, et al. 2020. J Clin Invest. 130:3483. PubMed Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed Gross KM, et al. 2019. Cell Rep. 28:394. PubMed Jatho A, et al. 2022. Cells. 11:. PubMed Fast EM, et al. 2021. Elife. 10:. PubMed |
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| RRID | AB_10639725 (BioLegend Cat. No. 108125) AB_10645327 (BioLegend Cat. No. 108126) |
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| Structure | Ly-6 multigene family, 18 kD |
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| Distribution | Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells |
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| Function | Regulates B and T cell responses |
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| Cell Type | B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells |
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| Biology Area | Immunology, Stem Cells |
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| Antigen References | 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709. |
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| Gene ID | 110454 |
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| UniProt | View information about Ly-6A/E on UniProt.org |
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| Clone | D7 |
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| Regulatory Status | RUO |
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| Other Names | Sca-1 |
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| Isotype | Rat IgG2a, κ |
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