Biolegend, 110716, APC/Cyanine7 anti-mouse CD45.1 Antibody
100microg
Catalog Number: 110716
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | SJL mouse thymocytes and splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions. |
| Concentration | 0.2 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)). |
| Application References(PubMed link indicates BioLegend citation) | Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed |
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| Product Citations | Jash A, et al. 2016. J Immunol. 197: 1159 - 1168. PubMed Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed Wang D, et al. 2022. Cell Rep. 39:110587. PubMed VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed Zhao M, et al. 2022. Nat Commun. 13:7600. PubMed Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed North K, et al. 2022. Nat Biotechnol. 40:1103. PubMed Morris V, et al. 2022. Cell Rep. 39:110752. PubMed Pulous FE, et al. 2022. Nat Neurosci. 25:567. PubMed Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Le Q, et al. 2021. Clin Cancer Res. Online ahead of prin. PubMed Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed Frame JM, et al. 2020. Developmental Cell. 55(2):133-149.e6. PubMed Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed Amor C, et al. 2020. Nature. 583:127. PubMed Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed Benechet AP, et al. 2019. Nature. 574:200. PubMed Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed Fu H, et al. 2020. Front Immunol. 11:595813. PubMed Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed Li J, et al. 2018. Immunity. 49:178. PubMed Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed Sun L, et al. 2021. Cell Reports. 35(9):109188. PubMed Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed De Simone G, et al. 2021. Immunity. :. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed Burgess M, et al. 2014. Blood. 124:3947. PubMed Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed Chen F, et al. 2022. Cell Rep. 38:110215. PubMed Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Wang X, et al. 2022. Elife. 11:. PubMed Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed Chen S, et al. 2018. Nat Commun. 9:5298. PubMed Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed |
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| RRID | AB_313504 (BioLegend Cat. No. 110715) AB_313505 (BioLegend Cat. No. 110716) |
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| Structure | Protein tyrosine phosphatase (PTP) family, 180-240 kD |
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| Distribution | All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice |
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| Function | Phosphatase, T and B cell activation |
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| Ligand/Receptor | Galectin-1, CD2, CD3, CD4 |
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| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851. |
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| Gene ID | 19264 |
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| UniProt | View information about CD45.1 on UniProt.org |
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| Clone | A20 |
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| Regulatory Status | RUO |
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| Other Names | T200, Ly-5.1, LCA |
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| Isotype | Mouse (A.SW) IgG2a, κ |
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