Biolegend, 110721, Pacific Blue™ anti-mouse CD45.1 Antibody
25microg
Catalog Number: 110721
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | SJL mouse thymocytes and splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)). |
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| Application References(PubMed link indicates BioLegend citation) | Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed |
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| Product Citations | Le DT, et al. 2023. iScience. 26:106059. PubMed Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed Cheng H, et al. 2023. Nat Metab. 5:314. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed Page N, et al. 2018. Immunity. 48:937. PubMed Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Lin G, et al. 2013. J Immunol. 190:4627. PubMed Liu X, et al. 2021. eLife. 0.416666666666667. PubMed Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed Li L, et al. 2010. J Immunol. 184:1728. PubMed Müller M, et al. 2021. Nat Commun. 12:7036. PubMed Wigton E, et al. 2016. J Leukoc Biol. 100: 143 - 153. PubMed Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed Cheng Y, et al. 2021. Cancer Cell. . PubMed Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed Kalinski AL, et al. 2020. Elife. 9:00. PubMed Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed Chen X, et al. 2021. Cell Rep. 37:109991. PubMed Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed Nadrah K, et al. 2015. PLoS One. 10: 0136061. PubMed Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed Holian C 2007. Am J Respir Cell Mol Biol. 37:729. PubMed Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed Florez MA, et al. 2020. Cell Rep. 33:108530. PubMed Mills R, et al. 2015. J Immunol. 194:3675. PubMed Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed Fiege JK, et al. 2019. J Immunol. 203(4):936-945. PubMed Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed Diao J, et al. 2018. J Immunol. 201:1306. PubMed Luo H, et al. 2019. Cell Rep. 26:945. PubMed Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed Wünsche P et al. 2018. Cell stem cell. 23(1):132-146 . PubMed Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed |
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| RRID | AB_492867 (BioLegend Cat. No. 110721) AB_492866 (BioLegend Cat. No. 110722) |
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| Structure | Protein tyrosine phosphatase (PTP) family, 180-240 kD |
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| Distribution | All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice |
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| Function | Phosphatase, T and B cell activation |
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| Ligand/Receptor | Galectin-1, CD2, CD3, CD4 |
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| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851. |
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| Gene ID | 19264 |
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| UniProt | View information about CD45.1 on UniProt.org |
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| Clone | A20 |
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| Regulatory Status | RUO |
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| Other Names | T200, Ly-5.1, LCA |
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| Isotype | Mouse (A.SW) IgG2a, κ |
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