Biolegend, 110724, Alexa Fluor® 700 anti-mouse CD45.1 Antibody
100microg
Catalog Number: 110724
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | SJL mouse thymocytes and splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)). |
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| Application References(PubMed link indicates BioLegend citation) | Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed |
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| Product Citations | Lucca L, et al. 2014. J Immunol. 193:3267. PubMed Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed Withers D, et al. 2009. J Immunol. 183:5079. PubMed van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed Garidou L, et al. 2012. J Virol. 86:7060. PubMed Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed Baldwin SL, et al. 2021. PLoS One. 16:e0247990. PubMed Park JH, et al. 2022. STAR Protoc. 3:101607. PubMed Huang W, et al. 2014. J Immunol. 193:2267. PubMed Semmrich M, et al. 2011. Mucosal Immunol. 0.3125. PubMed Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed Rengarajan S, et al. 2020. Cell Rep Med. :1. PubMed Zenke S, et al. 2020. Immunity. 52(2):313-327. PubMed Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed Baasch S, et al. 2021. Cell. . PubMed Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed Ng SS, et al. 2020. Nat Immunol. 21:1205. PubMed Tondini E, et al. 2022. NPJ Vaccines. 7:64. PubMed Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed Zhang J, et al. 2021. Nature. 590:457. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Li C, et al. 2018. Cell. 174:285. PubMed Yabas M, et al. 2011. PLoS One. 6:e26440. PubMed Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed Huang W, et al. 2014. J Leukoc Biol. 96:55. PubMed Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed Senatus L, et al. 2020. JCI Insight. 5:00. PubMed Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed Li J, et al. 2021. Cell Rep. 37:110124. PubMed Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed Eftychi C et al. 2019. Immunity. 51(2):367-380 . PubMed Swarnalekha N, et al. 2021. Sci Immunol. 6:. PubMed Schuran FA, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed |
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| RRID | AB_493732 (BioLegend Cat. No. 110723) AB_493733 (BioLegend Cat. No. 110724) |
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| Structure | Protein tyrosine phosphatase (PTP) family, 180-240 kD |
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| Distribution | All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice |
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| Function | Phosphatase, T and B cell activation |
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| Ligand/Receptor | Galectin-1, CD2, CD3, CD4 |
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| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851. |
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| Gene ID | 19264 |
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| UniProt | View information about CD45.1 on UniProt.org |
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| Clone | A20 |
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| Regulatory Status | RUO |
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| Other Names | T200, Ly-5.1, LCA |
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| Isotype | Mouse (A.SW) IgG2a, κ |
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