Biolegend, 110729, PE/Cyanine7 anti-mouse CD45.1 Antibody
25microg
Catalog Number: 110729
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | SJL mouse thymocytes and splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions. |
| Concentration | 0.2 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Excitation Laser | Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
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| Application Notes |
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| The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)). |
| Application References(PubMed link indicates BioLegend citation) | Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed |
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| Product Citations | Burghardt S, et al. 2013. J Immunol. 191:5574. PubMed Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed Pasqual G, et al. 2023. Methods Mol Biol. 2618:71. PubMed Giampaolo S, et al. 2023. iScience. 26:106234. PubMed Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed du Halgouet A, et al. 2023. Immunity. 56:78. PubMed Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed Zhou J, et al. 2019. Immunity. 50:403. PubMed Yu Y, et al. 2022. iScience. 25:105004. PubMed Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed Puiffe ML, et al. 2020. Front Immunol. 11:600012. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Kinder JM, et al. 2020. Cell Reports. 31(12):107784.. PubMed Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed Jia W, et al. 2021. Nat Commun. 12:2118. PubMed Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed Yue X, et al. 2019. Nat Commun. 10:2011. PubMed Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed Geng S, et al. 2021. JCI Insight. 6:. PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed Mansell E, et al. 2020. Cell Stem Cell. . PubMed Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed Ronsard L, et al. 2021. Front Immunol. 12:730471. PubMed Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed Subedi A, et al. 2021. Cell Stem Cell. :. PubMed Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed Severance AL, et al. 2022. iScience. 25:104400. PubMed Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed Reimer D, et al. 2020. Cell Reports. 32(6):108030. PubMed Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed Kwok T, et al. 2022. Front Aging. 3:838943. PubMed Kang YH, et al. 2019. Nat Commun. 10:912. PubMed Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed Ghezraoui H, et al. 2018. Nature. 560:122. PubMed Niven J, et al. 2019. Cell Rep. 28:21. PubMed Koide S, et al. 2022. iScience. 25:103603. PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed Wilkinson AC, et al. 2019. Nature. 571:117. PubMed Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed Barrigan L, et al. 2013. Infect Immun . 81:2028. PubMed Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed Subramanian S, et al. 2022. Nat Immunol. 23:458. PubMed Chen S, et al. 2018. Nat Commun. 9:5298. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed |
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| RRID | AB_1134170 (BioLegend Cat. No. 110729) AB_1134168 (BioLegend Cat. No. 110730) |
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| Structure | Protein tyrosine phosphatase (PTP) family, 180-240 kD |
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| Distribution | All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice |
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| Function | Phosphatase, T and B cell activation |
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| Ligand/Receptor | Galectin-1, CD2, CD3, CD4 |
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| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851. |
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| Gene ID | 19264 |
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| UniProt | View information about CD45.1 on UniProt.org |
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| Clone | A20 |
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| Regulatory Status | RUO |
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| Other Names | T200, Ly-5.1, LCA |
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| Isotype | Mouse (A.SW) IgG2a, κ |
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