Biolegend, 110750, Alexa Fluor® 594 anti-mouse CD45.1 Antibody
100microg
Catalog Number: 110750
Send an RFQ.
Category
Loading…
| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | SJL mouse thymocytes and splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-F - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes |
|---|
| The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)). |
| Application References(PubMed link indicates BioLegend citation) | Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed |
|---|
| Product Citations | Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed Fidler TP, et al. 2021. Nature. 592:296. PubMed Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed |
|---|
| RRID | AB_2617018 (BioLegend Cat. No. 110750) |
|---|
| Structure | Protein tyrosine phosphatase (PTP) family, 180-240 kD |
|---|
| Distribution | All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice |
|---|
| Function | Phosphatase, T and B cell activation |
|---|
| Ligand/Receptor | Galectin-1, CD2, CD3, CD4 |
|---|
| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers |
|---|
| Molecular Family | CD Molecules |
|---|
| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851. |
|---|
| Gene ID | 19264 |
|---|
| UniProt | View information about CD45.1 on UniProt.org |
|---|
| Clone | A20 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | T200, Ly-5.1, LCA |
|---|
| Isotype | Mouse (A.SW) IgG2a, κ |
|---|
