Biolegend, 112203, Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) Antibody
25microg
Catalog Number: 112203
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume.For immunohistochemistry on frozen tissue sections, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser |
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| Blue Laser (488 nm) |
| RRID | AB_3683139 (BioLegend Cat. No. 112203) AB_3683139 (BioLegend Cat. No. 112204) |
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| Structure | 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats. |
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| Distribution | Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types |
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| Function | Mediates calcium-independent homophilic cell-cell adhesion |
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| Interaction | CD326 displays hemophilic binding. |
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| Ligand/Receptor | CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM |
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| Antigen References | Borkowski TA, et al. 1996. Eur J Immunol. 26:110-4. Bergsagel PL, et al. 1992. J Immunol. 148:590-6. |
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| Gene ID | 17075 |
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| UniProt | View information about CD326 on UniProt.org |
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| Clone | W20119E |
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| Regulatory Status | RUO |
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| Other Names | EGP40, MIC18, TROP1, KSA |
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| Isotype | Rat IgG2b, κ |
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