Biolegend, 112804, Biotin anti-mouse CD119 (IFN-γ R α chain) Antibody
500microg
Catalog Number: 112804
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | Soluble recombinant mouse IFN-γR α chain protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1. The 2E2 antibody does not block the binding of IFN-γ to IFN-γ receptor. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112804) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). |
| Application References(PubMed link indicates BioLegend citation) | Bach EA, et al. 1995. Science 270:1215. |
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| Product Citations | Tang Q, et al. 2022. Mol Ther. 30:2709. PubMed Kiritsy MC, et al. 2021. Elife. 10:. PubMed Bruin A, et al. 2010. Blood. 116:2559. PubMed Cella M, et al. 2010. Proc Natl Acad Sci U S A. 107:10961. PubMed Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed DanDan Huang, Lang Bao 2016. J Microbiol Immunol Infect. 49:859-865. PubMed Ribechini E, et al. 2017. Blood Adv. 0.699305556. PubMed |
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| RRID | AB_2123476 (BioLegend Cat. No. 112803) AB_2123475 (BioLegend Cat. No. 112804) |
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| Structure | Ig superfamily, class II cytokine receptor family, associates with IFN-γ AF-1, 90 kD |
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| Distribution | T cells and B cells, NK cells, myeloid cells, fibroblasts, endothelial and epithelial cells |
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| Function | Initiates and regulates immune response |
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| Ligand/Receptor | IFN-γ |
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| Cell Type | B cells, Endothelial cells, Epithelial cells, Fibroblasts, NK cells, T cells |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Basu M, et al. 1988. P. Natl. Acad. Sci. USA 85:6282. 2. Codias EK, et al. 1990. J. Immunol. 144:2197. |
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| Gene ID | 15979 |
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| UniProt | View information about CD119 on UniProt.org |
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| Clone | 2E2 |
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| Regulatory Status | RUO |
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| Other Names | IFN-γRα , IFN-γ Receptor α chain, CDw119 |
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| Isotype | Armenian Hamster IgG |
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