Biolegend, 112904, Biotin anti-mouse CD120a (TNF R Type I/p55) Antibody
500microg
Catalog Number: 112904
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | E. coli-expressed extracellular domain of the mouse TNFRI protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | FC - Quality testedELISA Detection - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| Flow Cytometry: For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112904) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).ELISA Detection1: The biotinylated 55R-170 antibody is useful as a detection antibody when paired with the Purified 55R-286 antibody (Cat. No. 113002) as the capture antibody.Additional reported applications (for the relevant formats) include:immunoprecipitation1, and In vitro and In vivo receptor blocking1. The Ultra-LEAF™Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 112905 & 112906). |
| Application References(PubMed link indicates BioLegend citation) | Sheehan, K. C., et al. 1995. J. Exp. Med. 181:607. |
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| RRID | AB_313528 (BioLegend Cat. No. 112903) AB_313529 (BioLegend Cat. No. 112904) |
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| Structure | TNFR superfamily, 55 kD |
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| Distribution | Variety of cell types at low levels |
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| Function | Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation |
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| Ligand/Receptor | TNF-α, LT-α (TNF-β) |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Aggarwal, B. B., et al. 1985. Nature 318 665. 2. Chan, F. K.M., et al. 2000. Science 288:2351. 3. Loetscher, H., et al. 1990. Cell 61:351. 4. Rothe, J., et al. 1993. Nature 364:798. |
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| Gene ID | 21937 |
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| UniProt | View information about CD120a on UniProt.org |
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| Clone | 55R-170 |
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| Regulatory Status | RUO |
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| Other Names | TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A |
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| Isotype | Armenian Hamster IgG |
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