Biolegend, 114407, Alexa Fluor® 488 anti-mouse I-A
25microg
Catalog Number: 114407
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| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | C3H.SW splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity3, immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections4,5 and acetone fixed epidermal sheets6. |
| Application References(PubMed link indicates BioLegend citation) | Wall KA, et al. 1983. J. Immunol. 131:1056. (IP) Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. Ozato K, et al. 1981. J. Immunol. 126:317. Khanna A, et al. 2000. J. Immunol. 164:1346. (IHC) Nikolic B, et al. 1999. J. Immunol. 162:3402. (IHC) Meuter S, et al. 2007. Mol. Cell. Biol. 27:983. (IHC) PubMed Ryan AO, et al. 2011. J Exp Med. 208;1041. PubMed. Sicking EM, et al. 2012. J Am Soc Nephrol. 23:629. PubMed. |
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| RRID | AB_493144 (BioLegend Cat. No. 114407) |
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| Structure | ß chain of I-Ab MHC class II |
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| Distribution | B cell and activated T cells, APCs of H-2b mice |
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| Function | Antigen presentation |
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| Ligand/Receptor | CD3/TCR, CD4 |
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| Cell Type | Antigen-presenting cells, B cells, T cells, Tregs |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | MHC Antigens |
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| Antigen References | 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. |
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| Gene ID | 14961 |
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| UniProt | View information about I-Ab on UniProt.org |
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| Clone | 25-9-17 |
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| Regulatory Status | RUO |
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| Other Names | MHC class II |
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| Isotype | Mouse (C3H) IgG2a, κ |
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