Biolegend, 114408, Alexa Fluor® 488 anti-mouse I-A
100microg
Catalog Number: 114408
Send an RFQ.
Category
Loading…
| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | C3H.SW splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes |
|---|
| Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity3, immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections4,5 and acetone fixed epidermal sheets6. |
| Application References(PubMed link indicates BioLegend citation) | Wall KA, et al. 1983. J. Immunol. 131:1056. (IP) Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. Ozato K, et al. 1981. J. Immunol. 126:317. Khanna A, et al. 2000. J. Immunol. 164:1346. (IHC) Nikolic B, et al. 1999. J. Immunol. 162:3402. (IHC) Meuter S, et al. 2007. Mol. Cell. Biol. 27:983. (IHC) PubMed Ryan AO, et al. 2011. J Exp Med. 208;1041. PubMed. Sicking EM, et al. 2012. J Am Soc Nephrol. 23:629. PubMed. |
|---|
| RRID | AB_493144 (BioLegend Cat. No. 114407) |
|---|
| Structure | ß chain of I-Ab MHC class II |
|---|
| Distribution | B cell and activated T cells, APCs of H-2b mice |
|---|
| Function | Antigen presentation |
|---|
| Ligand/Receptor | CD3/TCR, CD4 |
|---|
| Cell Type | Antigen-presenting cells, B cells, T cells, Tregs |
|---|
| Biology Area | Immunology, Innate Immunity |
|---|
| Molecular Family | MHC Antigens |
|---|
| Antigen References | 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. |
|---|
| Gene ID | 14961 |
|---|
| UniProt | View information about I-Ab on UniProt.org |
|---|
| Clone | 25-9-17 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | MHC class II |
|---|
| Isotype | Mouse (C3H) IgG2a, κ |
|---|
