Biolegend, 115008, Alexa Fluor® 488 anti-mouse I-A
100microg
Catalog Number: 115008
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | (C3H x BALB/c)F1 mouse cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, and immunohistochemical staining of acetone-fixed frozen sections. Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s). |
| Application References(PubMed link indicates BioLegend citation) | Hiramine C, et al. 1995. Cell. Immunol. 160:157. Wang Z, et al. 2004. J. Immunol. 172:5924. Ma XT, et al. 2006. Cancer Research 66:1169. Norian LA and Allen PM. 2004. J. Immunol. 173:835. PubMed Tian C, et al. 2007. J. Immunol. 179:6762. |
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| RRID | AB_493147 (BioLegend Cat. No. 115008) |
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| Structure | MHC class II |
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| Distribution | B cell and activated T cells, APCs of H-2d mice |
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| Function | Antigen presentation |
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| Ligand/Receptor | CD3/TCR, CD4 |
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| Cell Type | Antigen-presenting cells, B cells, T cells, Tregs |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | MHC Antigens |
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| Antigen References | 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. Wall KA, et al. 1983. J. Immunol. 131:1056. 4. Ridgway WM, et al. 1998. J. Exp. Med. 188:2267. |
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| Gene ID | 14961 |
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| UniProt | View information about I-Ad on UniProt.org |
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| Clone | 39-10-8 |
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| Regulatory Status | RUO |
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| Other Names | MHC class II |
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| Isotype | Mouse (C3H.SW) IgG3, κ |
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