Biolegend, 115527, Alexa Fluor® 700 anti-mouse CD19 Antibody
25microg
Catalog Number: 115527
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Category
| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse CD19-expressing K562 human erythroleukemia cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunofluorescence7. |
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| Application References(PubMed link indicates BioLegend citation) | Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed |
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| Product Citations | Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Kwong Chung CK, et al. 2017. Immunology. 150:221. PubMed Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed Orvain C, et al. 2022. Arthritis Res Ther. 24:13. PubMed du Halgouet A, et al. 2023. Immunity. 56:78. PubMed Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed Patton T, et al. 2023. Cell Death Dis. 14:111. PubMed Xiao R, et al. 2022. J Immunol. 208:2109. PubMed Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed Li J, et al. 2010. J Immunol. 184:6142. PubMed Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed Boldison J, et al. 2020. Cell Mol Immunol. 17:843. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed Perry D, et al. 2012. J Immunol. 189:793. PubMed Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed Kenderes KJ, et al. 2018. Cell Rep. 24:824. PubMed Roy R, et al. 2022. Elife. 11:. PubMed Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed Aldrich AL, et al. 2021. The Journal of Immunology. 206(4):751-765. PubMed Doron I, et al. 2021. Cell. 184(4):1017-1031.e14. PubMed Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed Vogel AB, et al. 2021. Nature. 592:283. PubMed Li H, et al. 2020. Nature. 584:274. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Wu S, et al. 2022. BMC Immunol. 23:17. PubMed Giampazolias E, et al. 2021. Cell. . PubMed Holm J, et al. 2015. PLoS One. 10:125495. PubMed Kalafati L, et al. 2020. Cell. 183:771. PubMed Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed Matsuda T, et al. 2022. iScience. 25:105324. PubMed Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed Bennion BG, et al. 2019. J Virol. 93. PubMed Vijay R, et al. 2021. J Exp Med. 218:. PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Rinaldi S, et al. 2021. PLoS Pathog. 17:e1009533. PubMed Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed Cruz F, et al. 2016. Stem Cells Trans Med. 5: 488-499. PubMed Gorman M, et al. 2016. J Virol. 90: 8212 - 8225. PubMed Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed |
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| RRID | AB_493734 (BioLegend Cat. No. 115527) AB_493735 (BioLegend Cat. No. 115528) |
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| Structure | Ig superfamily, associates with CD21 and CD81, 95 kD |
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| Distribution | Pro-B cells to mature B cells (during development), follicular dendritic cells |
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| Function | Modulates B cell activation and differentiation |
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| Ligand/Receptor | CD21, CD81, Leu-13 |
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| Cell Type | B cells, Dendritic cells |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437. |
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| Gene ID | 12478 |
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| UniProt | View information about CD19 on UniProt.org |
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| Clone | 6D5 |
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| Regulatory Status | RUO |
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| Other Names | B4 |
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| Isotype | Rat IgG2a, κ |
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