Biolegend, 115552, Alexa Fluor® 594 anti-mouse CD19 Antibody
100microg
Catalog Number: 115552
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Category
| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse CD19-expressing K562 human erythroleukemia cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-F - Quality testedFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 5.0 μg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application Notes | Additional reported applications (for the relevant formats) include: immunofluorescence7. |
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| Application References(PubMed link indicates BioLegend citation) | Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed |
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| Product Citations | Schwarzenberg FL, et al. 2022. Front Immunol. 13:947961. PubMed Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed Rey–Suarez I, et al. 2020. Nat Commun. 0.763194444. PubMed Ogawa T, et al. 2022. PLoS One. 17:e0262397. PubMed Tuong ZK, et al. 2021. Cell Rep. 37:110132. PubMed |
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| RRID | AB_2563459 (BioLegend Cat. No. 115552) |
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| Structure | Ig superfamily, associates with CD21 and CD81, 95 kD |
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| Distribution | Pro-B cells to mature B cells (during development), follicular dendritic cells |
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| Function | Modulates B cell activation and differentiation |
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| Ligand/Receptor | CD21, CD81, Leu-13 |
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| Cell Type | B cells, Dendritic cells |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437. |
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| Gene ID | 12478 |
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| UniProt | View information about CD19 on UniProt.org |
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| Clone | 6D5 |
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| Regulatory Status | RUO |
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| Other Names | B4 |
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| Isotype | Rat IgG2a, κ |
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