Biolegend, 116111, Alexa Fluor® 488 anti-mouse CD54 Antibody
25microg
Catalog Number: 116111
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse NS-1 cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136). |
| Application References(PubMed link indicates BioLegend citation) | Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block) Horley KJ, et al. 1989. EMBO J. 8:2889. (Block) Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC) Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP) Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed |
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| Product Citations | Sarelius R 2010. J Immunol. 184:5242. PubMed Biram A, et al. 2020. Cell Rep. 30:1910. PubMed Hsieh C, et al. 2014. J Immunol. 193:3693. PubMed Bonnardel J, et al. 2019. Immunity. 51:638. PubMed Guilliams M, et al. 2022. Cell. 185:379. PubMed Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed Muradashvili N, et al. 2012. J Cereb Blood Flow Metab. 32:150. PubMed Sumagin R, et al. 2011. Am J Physiol Cell Physiol. 301:C804. PubMed Hsing CH, et al. 2021. Biomedicines. 9:. PubMed |
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| RRID | AB_493494 (BioLegend Cat. No. 116111) AB_493493 (BioLegend Cat. No. 116112) |
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| Structure | Ig superfamily, 90 kD |
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| Distribution | Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells |
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| Function | Immune reaction, inflammation, adhesion |
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| Ligand/Receptor | CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen |
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| Cell Type | B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells |
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| Biology Area | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1990. Nature 346:425. 3. Rothlein R, et al. 1986. J. Immunol. 137:1270. |
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| Gene ID | 15894 |
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| UniProt | View information about CD54 on UniProt.org |
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| Clone | YN1/1.7.4 |
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| Regulatory Status | RUO |
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| Other Names | ICAM-1, Ly-47 |
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| Isotype | Rat IgG2b, κ |
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