Biolegend, 116410, Alexa Fluor® 488 anti-mouse I-A
100microg
Catalog Number: 116410
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Category
| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | C57BL/10J splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5). Does not react with other haplotypes (e.g., d, f, q, r, s). |
| Application References(PubMed link indicates BioLegend citation) | Wall KA, et al. 1983. J. Immunol. 131:1056. (FC) Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC) Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF) Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF) Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC) Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF) Nakagawa TY, et al. 1999. Immunity 10:207. (IP) Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed. |
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| Product Citations | Shukla A, et al. 2021. Adv Nanobiomed Res. 1:. PubMed Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed Khare P, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.09.002. PubMed Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed |
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| RRID | AB_493140 (BioLegend Cat. No. 116410) |
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| Structure | MHC class II |
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| Distribution | B cell and activated T cells, APCs of H-2b mice |
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| Function | Antigen presentation |
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| Ligand/Receptor | CD3/TCR, CD4 |
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| Cell Type | Antigen-presenting cells, B cells, T cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | MHC Antigens |
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| Antigen References | 1. Watts C. 1997. Annu. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323. |
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| Gene ID | 14961 |
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| UniProt | View information about I-Ab on UniProt.org |
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| Clone | AF6-120.1 |
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| Regulatory Status | RUO |
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| Other Names | MHC class II |
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| Isotype | Mouse (BALB/c) IgG2a, κ |
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