Biolegend, 116509, Alexa Fluor® 488 anti-mouse H-2K
25microg
Catalog Number: 116509
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Category
| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | C57BL mouse splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections. Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v). |
| Application References(PubMed link indicates BioLegend citation) | Loken MR, et al. 1982. J. Immunol. Methods 50:R85. Wall KA, et al. 1983. J. Immunol. 131:1056. Andersson M, et al. 1998. J. Immunol. 161:6475. Shao H, et al. 2005. J. Immunol. 175:1851. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed |
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| Product Citations | Balakrishnan PB, et al. 2022. Nano Res. 15:2300. PubMed Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed Tsurutani N, et al. 2016. J Immunol. 196: 124 - 134. PubMed |
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| RRID | AB_492915 (BioLegend Cat. No. 116510) |
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| Structure | MHC class I |
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| Distribution | Nucleated cells of the H-2Kb haplotype |
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| Function | Antigen presentation |
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| Ligand/Receptor | CD3/TCR, CD8 |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | MHC Antigens |
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| Antigen References | 1. Watts C. 1997. Ann. Review Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369. |
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| Gene ID | 14972 |
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| UniProt | View information about H-2Kb on UniProt.org |
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| Clone | AF6-88.5 |
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| Regulatory Status | RUO |
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| Other Names | MHC class I |
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| Isotype | Mouse (BALB/c) IgG2a, κ |
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