Biolegend, 120112, Alexa Fluor® 488 anti-mouse CD197 (CCR7) Antibody
25microg
Catalog Number: 120112
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse CCR7 transfected RBL-2H3 cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures). |
| Application References(PubMed link indicates BioLegend citation) | Ohl L, et al. 2004. Immunity 21:279. Ritter U, et al. 2004. J. Leukocyte Biol. 76:472. Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC) Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC) Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed Kang SG, et al. 2007. J. Immunol. 179:3724. Mao A et al. 2005. J. Immunol. 175:5146. PubMed Allende ML, et al. 2008. FASEB J. 22:307. PubMed Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed Chen H, et al. 2005. J. Immunol. 175:591. PubMed Florido M, et al. 2009. Immunobiology. 214:643. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed |
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| Product Citations | Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Chen H, et al. 2005. J Immunol. 175:591. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Hwang I, Kehrl C 2004. J Immunol. 179:439. PubMed O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed Hu K, et al. 2013. J Immunol. 191:1935. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Tomaru U et al. 2019. Cell reports. 26(3):639-651 . PubMed |
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| RRID | AB_492842 (BioLegend Cat. No. 120112) AB_492841 (BioLegend Cat. No. 120110) |
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| Structure | Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD |
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| Distribution | Hematopoietic stem cells, T subsets, mature dendritic cells |
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| Function | Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis. |
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| Ligand/Receptor | MIP3 beta, SLC (6CKine, Exodus-2, TCA-4) |
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| Cell Type | Hematopoietic stem and progenitors, T cells, Dendritic cells, Tregs |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors, GPCR |
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| Antigen References | 1. Schweickart VL, et al. 1994. Genomics 23:643. 2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118. 3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053. 4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025. |
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| Gene ID | 12775 |
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| UniProt | View information about CD197 on UniProt.org |
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| Clone | 4B12 |
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| Regulatory Status | RUO |
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| Other Names | C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197 |
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| Isotype | Rat IgG2a, κ |
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