Biolegend, 120211, Alexa Fluor® 488 anti-mouse CD357 (GITR) Antibody
100microg
Catalog Number: 120211
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Recombinant mouse GITR protein and Transfected cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 120216). |
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| Application References(PubMed link indicates BioLegend citation) | Cobbold SP, et al. 2004. J. Immunol. 172:6003.Biburger M, et al. 2008. J. Leukocyte Biol. 84:264. PubMed |
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| Product Citations | Iype T, et al. 2010. J Immunol. 185:6115. PubMed |
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| RRID | AB_439727 (BioLegend Cat. No. 120211) |
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| Structure | TNF receptor superfamily member |
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| Distribution | Low levels on resting T cells, high on CD4+CD25+ Treg cells, expression upregulated upon activation. |
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| Function | Co-stimulate GITR expressing cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells. |
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| Ligand/Receptor | GITR ligand (TNFSF18) |
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| Cell Type | T cells, Tregs |
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| Biology Area | Immunology, Costimulatory Molecules |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:150592. Ronchetti S, et al. 2004. Eur. J. Immunol. 34:6133. Kanamaru F, et al. 2004. J. Immunol. 172:6134. Shimizu J, et al. 2002. Nat. Immunol. 3:1355. Stephens GL, et al. 2004. J. Immunol. 173:50086. McHugh RS, et al. 2002. Immunity 16:311 |
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| Gene ID | 21936 |
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| UniProt | View information about CD357 on UniProt.org |
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| Clone | YGITR 765 |
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| Regulatory Status | RUO |
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| Other Names | TNFRSF18, AITR |
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| Isotype | Rat IgG2b, κ |
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