Biolegend, 122017, Alexa Fluor® 488 anti-mouse CD28 Antibody
25microg
Catalog Number: 122017
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | A20 cells and recombinant mCD28Ig |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, and in vitro induction of thymocyte differentiation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 122004). |
| Application References(PubMed link indicates BioLegend citation) | Dennehy KM, et al. 2006. J. Immunol. 176:5725. |
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| RRID | AB_604065 (BioLegend Cat. No. 122017) |
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| Structure | Ig superfamily, 44 kD |
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| Distribution | Thymocytes, CD4+, CD8+ peripheral T cells, NK cells |
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| Function | Costimulates T and NK cells |
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| Ligand/Receptor | CD80 (B7-1), CD86 (B7-2) |
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| Cell Type | NK cells, T cells, Thymocytes, Tregs |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233. 3. Gross JA, et al. 1992. J. Immunol. 149:380. |
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| Gene ID | 12487 |
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| UniProt | View information about CD28 on UniProt.org |
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| Clone | E18 |
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| Regulatory Status | RUO |
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| Other Names | Tp44, T44 |
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| Isotype | Mouse IgG2b, κ |
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