Biolegend, 122515, Alexa Fluor® 488 anti-mouse Ly-6A/E (Sca-1) Antibody
25microg
Catalog Number: 122515
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | mouse pre-T cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7 |
| Application References(PubMed link indicates BioLegend citation) | Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD) Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC) van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP) van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF) Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Artinger EL, et al. 2013. PNAS. 110:12000. PubMed |
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| Product Citations | Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed Flaczyk A, et al. 2013. J Immunol. 191:2503. PubMed Li Q, et al. 2020. Am J Pathol. 190:2453. PubMed |
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| RRID | AB_756201 (BioLegend Cat. No. 122516) |
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| Structure | Ly-6 multigene family, 18 kD |
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| Distribution | Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells |
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| Function | Regulates B and T cell responses |
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| Cell Type | B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells |
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| Biology Area | Immunology, Stem Cells |
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| Antigen References | 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709. |
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| Gene ID | 110454 |
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| UniProt | View information about Ly-6A/E on UniProt.org |
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| Clone | E13-161.7 |
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| Regulatory Status | RUO |
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| Other Names | Sca-1 |
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| Isotype | Rat IgG2a, κ |
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