Biolegend, 123426, Alexa Fluor® 594 anti-mouse CD21/CD35 (CR2/CR1) Antibody
100microg
Catalog Number: 123426
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | CD35/CFA |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-F - Quality tested 3D IHC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5.0 µg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application References(PubMed link indicates BioLegend citation) |
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| Boackle S, et al. 2001 Immunity 15:775. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
| Product Citations | Yeh CH, et al. 2022. Immunity. 55:272. PubMed Nakata T, et al. 2020. Proc Natl Acad Sci U S A. 117:28930. PubMed Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed Bunse M, et al. 2021. Nat Commun. 0.666666667. PubMed |
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| RRID | AB_2632698 (BioLegend Cat. No. 123426) |
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| Structure | 145 kD/190 kD type I transmembrane glycoprotein |
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| Distribution | B cells, follicular dendritic cells |
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| Function | Signal transduction |
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| Ligand/Receptor | Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b. |
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| Cell Type | B cells, Dendritic cells |
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| Biology Area | Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157. |
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| Gene ID | 1290212946 |
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| UniProt | View information about CD21/CD35 on UniProt.org |
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| Clone | 7E9 |
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| Regulatory Status | RUO |
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| Other Names | CR2/CR1 |
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| Isotype | Rat IgG2a, κ |
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