Biolegend, 124909, Alexa Fluor® 488 anti-mouse DC Marker (33D1) Antibody
25microg
Catalog Number: 124909
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse dendritic cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunofluorescence3 and immunohistochemical staining of acetone-fixed frozen sections4. Binding of clone 33D1 is calcium-dependent. We recommend staining in EDTA-free FACS buffer containing a source of Ca2+ ions. |
| Application References(PubMed link indicates BioLegend citation) | Crowley MT, et al. 1990. J. Immunol. Methods 133:55. Nussenzwig MC, et al. 1982. P. Natl. Acad. Sci. USA 79:161. Steinman RM, et al. 1983. J. Exp. Med. 157:613. (IF) Fischer HG, et al. 2000. J. Immunol. 164:4826. (IHC) |
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| Product Citations | Ding Z, Dahlin J 2016. Sci Rep. 6:28290. PubMed |
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| RRID | AB_1186125 (BioLegend Cat. No. 124910) |
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| Structure | The antigen recognized by 33D1 is yet unknown. |
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| Distribution | 33D1 antigen was reported on dendritic cell populations from mouse thymus, spleen lymph nodes and Peyer patches. Bone marrow dendritic cells can be induced to express 33D1 antigen in the presence of GM-CSF. In mouse spleen, most N418+ cells are also 33D1 positive. |
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| Function | GM-CSF is reported to increase expression of 33D1 antigen on dendritic cells from bone marrow cells and IL-4 reported to down regulate the 33D1 antigen. |
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| Cell Type | Dendritic cells |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules |
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| Gene ID | NA |
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| UniProt | View information about 33D1 on UniProt.org |
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| Clone | 33D1 |
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| Regulatory Status | RUO |
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| Other Names | Dendritic Cell Marker, 33D1, DCIR2 (dendritic cell inhibitory receptor 2) |
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| Isotype | Rat IgG2b, κ |
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