Biolegend, 126541, Alexa Fluor® 488 anti-mouse CD183 (CXCR3) Antibody
25microg
Catalog Number: 126541
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | Mouse N-terminus of CXCR3 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered. |
| Application References(PubMed link indicates BioLegend citation) | Krug A, et al. 2002. J. Immunol. 169:6079. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed Barbi J, et al. 2009. FASEB J. 23:3990. PubMed Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed Uppaluri R, et al. 2008. Transplantation 86:137. |
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| Product Citations | Chen RJ, et al. 2022. iScience. 25:105595. PubMed |
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| RRID | AB_2650831 (BioLegend Cat. No. 126541) AB_2650832 (BioLegend Cat. No. 126542) |
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| Distribution | T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors |
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| Function | Essential in T cell recruitment to sites of inflammation |
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| Cell Type | Dendritic cells, Hematopoietic stem and progenitors, T cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors, GPCR |
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| Gene ID | 12766 |
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| UniProt | View information about CD183 on UniProt.org |
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| Clone | CXCR3-173 |
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| Regulatory Status | RUO |
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| Other Names | CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R ) |
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| Isotype | Armenian Hamster IgG |
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