Biolegend, 126627, Alexa Fluor® 488 anti-mouse CD8b (Ly-3) Antibody
25microg
Catalog Number: 126627
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse thymocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality testedIHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections. |
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| Application References(PubMed link indicates BioLegend citation) | McNical AM, et al. 2007. Eur. J. Immunol. 37:1634. |
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| Product Citations | Peymanfar Y, et al. 2023. Int J Mol Sci. 24:. PubMed Ozga AJ, et al. 2022. Immunity. 55:82. PubMed Blanas A, et al. 2022. iScience. 25:104719. PubMed van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed |
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| RRID | AB_2800618 (BioLegend Cat. No. 126627) AB_2800619 (BioLegend Cat. No. 126628) |
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| Structure | Ig superfamily, CD8β chain, 34 kD |
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| Distribution | Majority of thymocytes, cytotoxic T cell subsets |
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| Function | Co-receptor of TCR |
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| Ligand/Receptor | MHC class I |
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| Cell Type | T cells, Thymocytes, Tregs |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press. 2. Zamoyska R. 1994. Immunity 1:243-246. 3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523. 4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. |
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| Gene ID | 12526 |
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| UniProt | View information about CD8beta on UniProt.org |
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| Clone | YTS156.7.7 |
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| Regulatory Status | RUO |
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| Other Names | Lyt-3, Ly-3 |
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| Isotype | Rat IgG2b, κ |
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