Biolegend, 127012, Alexa Fluor® 488 anti-mouse CD317 (BST2, PDCA-1) Antibody
100microg
Catalog Number: 127012
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse plasmacytoid dendritic cells (DCs) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. |
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| Application References(PubMed link indicates BioLegend citation) | Blasius AL, et al. 2006. J. Immunol. 177:3260. Schliemann C, et al. 2010. Blood 115:736. (FA, IF) Rajagopal D, et al. 2010. Blood 115:1949. (Depletion) Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion) Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed |
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| Product Citations | Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed Xiao Y, et al. 2021. Cell. 184:6037. PubMed Kim H, et al. 2015. Cytotherapy. . PubMed Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed |
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| RRID | AB_1953287 (BioLegend Cat. No. 127012) |
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| Structure | Type II transmembrane glycoprotein with a molecular mass of 29-33 kD. |
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| Distribution | Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma. |
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| Function | Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells. |
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| Cell Type | Dendritic cells |
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| Biology Area | Costimulatory Molecules, Immunology, Innate Immunity |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Douglas JL. et al. 2009. J Virol. 83(16):7931 2. Cao W et al. 2009. J. Exp. Med. 206(7):1603 3. Neil SJ. et al. 2008. Nature 451:425 |
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| Gene ID | 69550 |
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| UniProt | View information about CD317 on UniProt.org |
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| Clone | 927 |
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| Regulatory Status | RUO |
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| Other Names | BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1 |
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| Isotype | Rat IgG2b, κ |
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