Biolegend, 127226, Alexa Fluor® 594 anti-mouse CD73 Antibody
100microg
Catalog Number: 127226
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| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | CHO cells transfected with mouse CD73 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-F - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application References(PubMed link indicates BioLegend citation) |
|---|
| Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P) |
| RRID | AB_2728144 (BioLegend Cat. No. 127225) AB_2728145 (BioLegend Cat. No. 127226) |
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| Structure | 69 kD GPI-anchored surface protein |
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| Distribution | Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73 |
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| Function | A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates |
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| Ligand/Receptor | Nucleoside 5' monophosphate |
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| Cell Type | B cells, Mesenchymal Stem Cells, Tregs |
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| Biology Area | Costimulatory Molecules, Immunology, Stem Cells |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95. |
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| Regulation | Expression is regulated by the development and maturation of leukocytes |
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| Gene ID | 23959 |
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| UniProt | View information about CD73 on UniProt.org |
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| Clone | TY/11.8 |
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| Regulatory Status | RUO |
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| Other Names | Ecto-5'-nucleotidase |
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| Isotype | Rat IgG1, κ |
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