Biolegend, 127414, Alexa Fluor® 594 anti-mouse Podoplanin Antibody
100microg
Catalog Number: 127414
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Syrian Hamster |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality testedIHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 10 μg/ml is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9. |
| Application References(PubMed link indicates BioLegend citation) | Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. Farr AG, et al. 1992. J. Exp. Med. 176:1477. Bekiaris V, et al. 2008. J. Immunol. 180:6768. Algars A, et al. 2011. Blood 117:4387. PubMed Reis VO, et al. 2012. Immunobiology. 217:831. PubMed Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC) Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed Bunse M, et al. 2021. Nat Commun. 0.666666667. PubMed |
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| RRID | AB_2563351 (BioLegend Cat. No. 127414) |
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| Structure | 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein. |
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| Distribution | Expressed on epithelial and mesothelial cells. |
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| Cell Type | Epithelial cells |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers |
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| Antigen References | 1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. 2. Schacht V, et al. 2005. Am. J. Pathol. 166:913. |
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| Gene ID | 14726 |
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| UniProt | View information about Podoplanin on UniProt.org |
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| Clone | 8.1.1 |
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| Regulatory Status | RUO |
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| Other Names | T1a, gp38, stromal cell marker |
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| Isotype | Syrian Hamster IgG |
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