Biolegend, 127603, Biotin anti-mouse Ly-6G Antibody
50microg
Catalog Number: 127603
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Pack Size6 g
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Ly-6G transfected EL-4J cell line. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | FC - Quality testedIHC - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416. Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662). |
| Application References(PubMed link indicates BioLegend citation) | Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC) Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC) Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC) Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC) Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC) Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC) Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete) Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete) Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete) Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed Ma C, et al. 2012. J. Leukoc. Biol. 92:1199. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Barkaway A, et al. 2021. Immunity. . PubMed Haffner-Luntzer M, et al. 2017. Eur J Med Res. 10.1186/s40001-017-0264-y. PubMed Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed Xu H, et al. 2023. Elife. 12:. PubMed Song R, et al. 2022. Immunology. 165:274. PubMed Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Gargaro M, et al. 2022. Immunity. 55:1032. PubMed Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed Davis FM, et al. 2020. J Immunol. 2503:204. PubMed Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed Blazek K, et al. 2015. J Exp Med. 212:845. PubMed Haffner–Luntzer M, et al. 2019. Proc Natl Acad Sci U S A. 116:8615. PubMed Murata A, et al. 2020. Front Immunol. 11:775. PubMed Sasse C, et al. 2022. Pathogens. 11:. PubMed de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed Tang Y, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G156 - G165. PubMed Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed Periasamy S, et al. 2016. PLoS Pathog. 12: 1005517. PubMed Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed Lerman I, et al. 2019. Mol Cancer Res. 17:845. PubMed Evans R, et al. 2019. Cell Rep. 27:1967. PubMed Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed Best SA, et al. 2018. Cell Metab. 27:935. PubMed Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed Vaibhav K, et al. 2018. J Exp Med. 215:2636. PubMed Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Moorlag SJCFM, et al. 2020. Cell Rep. 33:108387. PubMed Ryzhov S, et al. 2011. J Immunol. 187:6120. PubMed Chauveau A, et al. 2020. Immunity. 52:794. PubMed Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed Muthukrishnan G, et al. 2021. Frontiers in Immunology. 12:651515. PubMed Popova T, et al. 2016. PLoS One. 11: 0163163. PubMed Steiger S, et al. 2015. J Immunol. 195: 5495 - 5502. PubMed Yang L, et al. 2012. J Immunol. 189:3741. PubMed Chen Z, et al. 2021. Front Immunol. 12:641874. PubMed Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed Kalafati L, et al. 2020. Cell. 183:771. PubMed Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed Mohammadpour H, et al. 2021. Cell Rep. 37:109883. PubMed Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed Sahakian E, et al. 2017. J Leukoc Biol. 102:475. PubMed Anderson DA, et al. 2022. J Exp Med. 219:. PubMed Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Huang X, et al. 2021. Immunity. . PubMed Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed Morita Y, et al. 2022. Bone Res. 10:56. PubMed De Cock J, et al. 2016. Cancer Res . 76(23):6778-6784. PubMed Cai W, et al. 2019. J Neuroinflammation. 0.788194444. PubMed Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed Liao YC, et al. 2021. Front Immunol. 12:743030. PubMed Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed |
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| RRID | AB_1186105 (BioLegend Cat. No. 127603) AB_1186108 (BioLegend Cat. No. 127604) |
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| Structure | A 21-35 kD GPI-anchorded membrane protein |
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| Distribution | Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C. |
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| Cell Type | Granulocytes, Macrophages, Monocytes |
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| Biology Area | Immunology, Innate Immunity |
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| Antigen References | Fleming TJ, et al. 1993. J. Immunol. 151:2399. |
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| Gene ID | 546644 |
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| UniProt | View information about Ly-6G on UniProt.org |
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| Clone | 1A8 |
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| Regulatory Status | RUO |
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| Other Names | Lymphocyte antigen 6 complex, locus G |
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| Isotype | Rat IgG2a, κ |
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