Biolegend, 127604, Biotin anti-mouse Ly-6G Antibody
500microg
Catalog Number: 127604
Send an RFQ.
Pack Size6 g
Loading…
| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Ly-6G transfected EL-4J cell line. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | FC - Quality testedIHC - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes |
|---|
| While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416. Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662). |
| Application References(PubMed link indicates BioLegend citation) | Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC) Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC) Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC) Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC) Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC) Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC) Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete) Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete) Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete) Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed Ma C, et al. 2012. J. Leukoc. Biol. 92:1199. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
|---|
| Product Citations | Barkaway A, et al. 2021. Immunity. . PubMed Haffner-Luntzer M, et al. 2017. Eur J Med Res. 10.1186/s40001-017-0264-y. PubMed Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed Xu H, et al. 2023. Elife. 12:. PubMed Song R, et al. 2022. Immunology. 165:274. PubMed Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Gargaro M, et al. 2022. Immunity. 55:1032. PubMed Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed Davis FM, et al. 2020. J Immunol. 2503:204. PubMed Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed Blazek K, et al. 2015. J Exp Med. 212:845. PubMed Haffner–Luntzer M, et al. 2019. Proc Natl Acad Sci U S A. 116:8615. PubMed Murata A, et al. 2020. Front Immunol. 11:775. PubMed Sasse C, et al. 2022. Pathogens. 11:. PubMed de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed Tang Y, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G156 - G165. PubMed Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed Periasamy S, et al. 2016. PLoS Pathog. 12: 1005517. PubMed Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed Lerman I, et al. 2019. Mol Cancer Res. 17:845. PubMed Evans R, et al. 2019. Cell Rep. 27:1967. PubMed Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed Best SA, et al. 2018. Cell Metab. 27:935. PubMed Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed Vaibhav K, et al. 2018. J Exp Med. 215:2636. PubMed Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Moorlag SJCFM, et al. 2020. Cell Rep. 33:108387. PubMed Ryzhov S, et al. 2011. J Immunol. 187:6120. PubMed Chauveau A, et al. 2020. Immunity. 52:794. PubMed Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed Muthukrishnan G, et al. 2021. Frontiers in Immunology. 12:651515. PubMed Popova T, et al. 2016. PLoS One. 11: 0163163. PubMed Steiger S, et al. 2015. J Immunol. 195: 5495 - 5502. PubMed Yang L, et al. 2012. J Immunol. 189:3741. PubMed Chen Z, et al. 2021. Front Immunol. 12:641874. PubMed Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed Kalafati L, et al. 2020. Cell. 183:771. PubMed Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed Mohammadpour H, et al. 2021. Cell Rep. 37:109883. PubMed Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed Sahakian E, et al. 2017. J Leukoc Biol. 102:475. PubMed Anderson DA, et al. 2022. J Exp Med. 219:. PubMed Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Huang X, et al. 2021. Immunity. . PubMed Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed Morita Y, et al. 2022. Bone Res. 10:56. PubMed De Cock J, et al. 2016. Cancer Res . 76(23):6778-6784. PubMed Cai W, et al. 2019. J Neuroinflammation. 0.788194444. PubMed Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed Liao YC, et al. 2021. Front Immunol. 12:743030. PubMed Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed |
|---|
| RRID | AB_1186105 (BioLegend Cat. No. 127603) AB_1186108 (BioLegend Cat. No. 127604) |
|---|
| Structure | A 21-35 kD GPI-anchorded membrane protein |
|---|
| Distribution | Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C. |
|---|
| Cell Type | Granulocytes, Macrophages, Monocytes |
|---|
| Biology Area | Immunology, Innate Immunity |
|---|
| Antigen References | Fleming TJ, et al. 1993. J. Immunol. 151:2399. |
|---|
| Gene ID | 546644 |
|---|
| UniProt | View information about Ly-6G on UniProt.org |
|---|
| Clone | 1A8 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Lymphocyte antigen 6 complex, locus G |
|---|
| Isotype | Rat IgG2a, κ |
|---|
